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KMID : 0545120070170091550
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 9 p.1550 ~ p.1553
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Functional Characteristics of Cyclodextrin Glucanotransferase from Alkalophilic Bacillus sp. BL-31 Highly Specific for IntermolecularTransglycosylation of Bioflavonoids
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Go Young-Hoon
Kim Tae-Kwon
Lee Kwang-Woo
Lee Yong-Hyun
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Abstract
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The functional characteristics of a β-cyclodextrin glucanotransferase (CGTase) excreted from alkalophilic Bacillus sp. BL-31 that is highly specific for the intermolecular transglycosylation of bioflavonoids were investigated. The new β-CGTase showed high specificities for glycosyl acceptor bioflavonoids, including naringin, rutin, and hesperidin, and especially naringin. The transglycosylation of naringin into glycosyl naringin was then carried out under the conditions of 80 units of CGTase per gram of maltodextrin, 5 g/l of naringin, 25 g/l of maltodextrin, and 1 mM Mn2+ ion at 40oC for 6 h, resulting in a high conversion yield of 92.1%.
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KEYWORD
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Cyclodextrin glucanotransferase, alkalophilic Bacillus sp, BL-31, intermolecular transglycosylation, bioflavonoids, glycosyl naringin
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